The text, “Analysis of the
Mycotoxin Patulin in Apple Juice Using the Flexar FX-15 UHPLC-UV”, Padmaja
Prabhu (2011), describes a rapid method for the quantification and the
identification of Patulin in apple juice. Patulin is produced by a variety of
molds. Patulin is not easy to eliminate, because the Patulin migrates in the
flesh. Patulin has multiple negative affects
within the human cells, the maximum concentration is 50μg
of Patulin/kg apple juice. The method that is explained in this text uses high
performance liquid chromatography with UV detection. In this experiment there were
a number of apple juice samples analyzed.
The samples were analyzed by using
an Ultra High Performance Liquid Chromatograph with a UV/Vis detector. At first
there was a stock solution prepared, which consisted of Patulin dissolved in
ethyl acetate and a stock solution which consisted HMF in ethyl acetate. The
stock solution was made to make a calibration curve for the UHPLC and the
UV/vis. After this the samples were prepared and analyzed. Of each sample 25 μL
was injected in to the UHPLC.
Because HMF elutes very close to
Patulin, the separation between HMF and Patulin was demonstrated. Ethyl acetate
was used to dissolve Patulin and the extracts were treated with sodium sulphate
to eliminate moisture. The treatment was important because Patulin may be
destroyed if ethyl acetate is evaporated. Patulin is not stable at a high
temperature so a temperature of 40˚C was used.
The concentration of Patulin was
analyzed. The concentration of all the samples were less than 5μg/L.
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